Identification and photochemical tests of fungi mushrooms and honey specially Bangka Belitung

Pelawan bitter honey and Pelawan mushrooms are two examples of Bangka's unique biodiversity found in Central Bangka Regency. Pelawan mushroom grows in the Bangka Belitung Islands Province, is generally used as a food ingredient, and has the potential to be developed as a source of natural immunomodulators. Phenolic compounds are known to have immunomodulatory activity that can enhance the immune system. In addition, based on preliminary studies, honey is also used by local people to strengthen their immune systems. The purpose of this study was to identify the content of active compounds in Pelawan honey and Pelawan mushrooms. This was an experimental study with multiple stages of testing, including phytochemical screening, thin-layer chromatography testing, and extract standardization testing. The results of the study proved that the phytochemical test of Pelawan honey contained Flavonoid and Alkaloid compounds, and for the Thin Layer Chromatography (TLC) test, it contained Flavonoids and Alkaloids. The Pelawan mushroom phytochemical test contained phenolic and alkaloids with Mayer's reagent; the Pelawan mushroom thin layer chromatography test contained flavonoids with an average Rf value of 0.56, while the standardization of Pelawan mushroom extract produced a total ash content of 28%, drying shrinkage of 40.88 g/mL, specific gravity of 0.67 g/mL, water-soluble essence of 59.1%, and ethanolsoluble essence content of 41.12%. From these results, it could be surmised that honey and fighting fungi have the potential to be developed as immunomodulatory agents.