Utilization of tofu dregs waste as an alternative medium bacterial growth Pseudomonas aeruginosa atcc 27853

The high price of media and the lack of awareness related to the potential economic value of tofu dregs waste encouraged researchers to find innovations in the use of tofu dregs waste into alternative media products for bacterial growth. This study aims to determine the ability of tofu dregs alternative media in growing Pseudomonas aeruginosa ATCC 27853 bacteria and analyze the number of colonies and characteristics of Pseudomonas aeruginosa ATCC 27853 in tofu dregs alternative media. The research was conducted using the laboratory experiment method using the concentration level of tofu dregs media of 2%,3%,4%, and 5%. The results of the calculation of the number of colonies obtained the number of colonies of Pseudomonas aeruginosa ATCC 27853 on tofu dregs media concentrations of 2%,3%,4%, and 5% was (68; 94; 142; 156). The characteristics of pseudomonas aeruginosa colonies ATCC 27853 on tofu dregs alternative media are round, green, notched, 2 mm, convex, and wet. The observational characteristics of Gram staining obtained Gram-negative, bacillus, monobasil, red. Based on the Anova test, the results of 0.002 (P<0.005). Ha received showed a growth of the bacterium Pseudomonas aeruginosa ATCC 27853 on alternative media of 2%,3%,4%, and 5% tofu dregs. Following up with the Duncan test, the minimum tofu dreg concentration that can grow the bacteria Pseudomonas aeruginosa ATCC 27853 is a concentration of 2%. However, the optimum concentration of tofu dregs media that is good for the growth of Pseudomonas aeruginosa bacteria in this research was a concentration of 5%.