The black rice ethanol extract effect on polymorphonuclear cells proliferation of rats induced by lipopolysaccharide

Inflammation is a defense that exists in the body when an infection occurs. Excessiveinflammatory reactions can be induced by lipopolysaccharides (LPS) which are the mostimmunogenic components of a bacterial capsule or cell wall, which will result in damagingeffects on the body. Black rice (Oryza sativa L.indica) is one of the natural ingredientsthat contains phenols, flavonoids, and antioxidant anthocyanin as immunomodulator. Thisstudy aims to find out the effect of giving black rice ethanol extract as an anti inflammatory totheproliferationofpolymorphonuclearcells(PMN)inthespleenof ratsinducedon lipopolysaccharides. This study was conducted using 27 mice who were givenblack rice ethanol extract as much as 400 mg/kg BW in 14 days and then rats induced by LPS0.3 mg/kg BW at 24 hours intraperitoneally before being CO 2euthanized and taken the spleento be checked for measuring the size of the germinal center diameter. The observations showed the results of the germinal center diameter size in the treatment group with a value of1,311 ± 0.226 μm (p<0.05 compared to positive control group which showed that LPSadministration led to the disorganization of the white pulp and the reduction of lymphocytecells and PMN in the germinal center. In conclusion, black rice ethanol extract at a doseof 400 mg/kgBB can affect the proliferation of PMN indicated by changes in themorphology of the white pulp in the germinal center in the spleen of rats.