Metabolite profiling of Indonesian cacao using gas chromatography mass spectrometry

Devy, Lukita and Susilo, Agung Wahyu and Wachjar, Ade and Sobir, Sobir (2019) Metabolite profiling of Indonesian cacao using gas chromatography mass spectrometry. In: 6th International Conference on Sustainable Agriculture, Food and Energy.

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Indonesia as the world third largest cacao producer is also well known for its fine
cacao from Trinitario group. Fine cacao breeding will support the sustainability of Indonesian
specialty cocoa products. However, information on the distinction between metabolite profile of
Indonesian fine and bulk cacao are still limited. The objective of this study was to compare the
metabolite profile of Indonesian fine and bulk cacaos using Gas Chromatography-Mass
Spectrometry (GC-MS) method with methanol-formic acid-water extraction. Four Indonesian
fine cacaos (i.e. DR 2, DRC 16, PNT 16, PNT 17) and bulk cacaos (i.e. MCC 02, KW 516, KW
617, SUL 01) were used. Measurements were conducted on untargeted metabolite profile,
theobromine (T), caffeine (C), T/C and total fat content. Forty-nine peaks were detected and 23
putative metabolites with high reliability were identified. Principal component analysis separated fine and bulk cacaos into two different groups. Hierarchical cluster analysis showed fine cacaos contained higher caffeine while bulk cacaos contained higher fatty acids (palmitic acid, stearic
acid and oleic acid) content than the opposite. However only fatty acids, which represented in total fat content, showed stable result under targeted analysis. Therefore, these metabolites could be further analysed as biomarker for fine cacao selection.

Item Type: Conference or Workshop Item (Paper)
Additional Information: DDC'23: 633.7 IOP Conference Series: Earth and Environmental Science volume 347
Uncontrolled Keywords: Cacao; Gas chromatography; Agricultural product; Chromatograph; Metabolite analysis
Subjects: Agriculture & Food
Agriculture & Food > Agricultural Chemistry
Agriculture & Food > Food Technology
Depositing User: Rasty -
Date Deposited: 13 Oct 2022 05:09
Last Modified: 13 Oct 2022 05:09

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