Thinkful of Icariin Induces Triple-Negative Breast Cancer Cell Apoptosis and Suppresses Invasion by Inhibiting the JNK/c-Jun Signaling Pathway [Letter]

This study used in silico and in vitro testing for future studies that will be followed up with in vivo tests to ensure that the proper dose is obtained while not being hazardous to normal or stem cells. Evaluation of the expression of gene role in apoptosis using RT-qPCR to Hs 578T and MDA-MB-468 cells treated with ICA can be performed to confirm the ICA induces development of apoptosis pathway via the extrinsic or intrinsic pathway. The development of apoptosis-targeting anticancer drugs has gained much interest since cell death induced by apoptosis causes minimal inflammation and gene expression is a method usually use to evaluate profile regulation which play role in the diseases such as diabetes, Alzheimer,4 etc. In addition to doses, repeated administration can also be considered in vivo trials. A prior study has undertaken ICA injection in rats with tumors, the use of non-human primate/NHP animal models can be explored considering the genetic similarities of NHP to humans.4,5