Toxicological Impact of Zinc Oxide Nanoparticles on Hybrid Grouper (Epinephelus fuscoguttatus x Epinephelus lanceolatus)

The use of zinc oxide nanoparticles (ZnO-NPs) across various industries hasraised concerns about their potential toxic effects on aquatic organisms,including hybrid grouper. The study aimed to evaluate the toxicological effectsof ZnO-NPs exposure on hybrid grouper (±20g) through the analysis ofmortality rate, blood performance, organ histopathology, and changes inimmune response. The methods involved exposure of ZnO-NPs in fourtreatment groups (i.e., 0, 50, 100, and 200 ppm) for 96 hours, followed byanalyzing the hematology, histopathology, and TNF-α, IL-1β antibody usingflow cytometry. The obtained data were analyzed using ANOVA. The resultsindicated that ZnO-NPs significantly increased fish mortality up to 50% at200ppm concentration. Significant changes were recorded in hematology,including a 62.96% reduction in hematocrit and a 20.28% increase in leukocytecount. Histopathological analysis showed severe intestinal tissue damage levelsreaching 40.43%. Enhanced immune activity expressing TNF-α and IL-1βantibodies, which increased by 15.08% and 6.81%. The highest accumulation ofZnO-NPs was found in the gill organs, with a concentration of 2.94 ppm. Thisstudy highlights the toxic risk of ZnO-NPs to hybrid grouper, emphasizing theneed for strict monitoring and the development of safety standards to supportsafer and more sustainable aquaculture practices