A validated HPLC-DAD method and comparison of different extraction techniques for analysis of scopoletin in noni-based products

The growth of the production of noni-based products due to the therapeutic effects and health benefits of noni fruits has led to the necessity for quality control and authentication to maintain product quality. This study proposes high-performance liquid chromatography with diode-array detector (HPLC-DAD) analysis for qualitative and quantitative analysis of scopoletin as a quality marker in noni products. Four extraction methods were compared and evaluated in the present study to extract scopoletin from noni products. The results showed that accelerated solvent extraction (ASE) yielded the highest scopoletin in the shortest time with superior relative standard deviation (RSD) value among other extraction methods investigated in this study (maceration, ultrasound, and microwave-assisted extraction). The established method demonstrated excellent linearity (R2 0.9995) within the range of 0.5–100 ​μg/mL, with adequate sensitivity (LOD 0.29 ​μg/mL). It also exhibited satisfactory precision and accuracy, represented by intra- and inter-day RSD values of less than 2% and recovery test results of 99.88%. The optimized and validated ASE-HPLC-DAD method has also been successfully applied to assess scopoletin content in a broad range of commercially available noni products in Indonesia. This includes 11 noni capsules, 5 instant powders, 3 teas, and 1 effervescence. It provides a reliable, fast, and simple alternative method with high accuracy and precision that can be further developed for quality control assessment and authentication of noni-based products.