Identifikasi Molekuler, Analisis Profil Asam Amino, dan Asam Lemak dari Beberapa Teripang Asal Perairan Indonesia

Sea cucumber is an important Indonesian commodity with various health benefits. However, the reports on their nutrition content are still limited. This study aimed to identify several Indonesian sea cucumbers and determine their amino acid and fatty acid profiles. The fresh sea cucumbers were obtained from Lampung, Gorontalo, North Maluku, and Bali. Species identification was carried out using Polymerase Chain Reaction (PCR) method. The sequencing results were matched to the Basic Local Alignment Search Tools (BLAST) database. The determination of amino acid and fatty acid contents was performed using Ultra-High-Performance Liquid Chromatography (UPLC) and Gas Chromatography-Flame Ionization Detector (GC-FID). Alolo, cera merah, lolosong, cera hitam, and pasir were identified as Bohadschia marmorata, Holothuria edulis, H. impatiens, H. atra, and H. scabra (wild and cultured), respectively. Non-essential amino acids (NEAA) in the samples were dominated by glycine and glutamic acid. The highest Essential Amino Acid (EAA) was threonine. H. edulis contained the highest total amino acid, i.e., 8.87 g/100 g. The lysine/arginine ratios in all samples were <1.00. The highest total fatty acid was wild and cultured H. scabra (0.41%). The fatty acids of H. atra, wild H. scabra, and B. marmorata were dominated by saturated fatty acid (SFA). In contrast, in the cultured H. scabra, H. edulis, and H. impatiens were dominated by polyunsaturated fatty acid (PUFA)/SFA. The PUFA/SFA ratio of H. edulis and H. impatiens was >1.00, while the H. atra, wild H. scabra, and B. marmorata were <1.00. Based on this result, it is concluded that H. edulis and H. impatiens are potential for nutraceutical ingredients.