A Response to Article “Anti-Diarrheal Activity of Hydromethanolic Crude Extract and Solvent Fractions of Acacia Seyal (Fabaceae) Roots in Mice” [Letter]

We were interested in reviewing the study performed and reported by Mengesha et al, regarding the evaluation of anti-diarrheal activity of the extract of Acacia seyal root tested in mice.1 Interestingly, according to our searches, there werestill very limited studies regarding Acacia seyal and most of those utilized its gums and bark for its antioxidant,antibacterial, cytotoxic till antimammary activities of Acacia seyal. 2–4 This study was likely to be the first evaluationstudy of the anti-diarrheal activity of the hydromethanolic extract of Acacia seyal, especially and specifically utilizing itsroot. Following the traditional method of organic solvent extraction, the hydromethanolic crude extract of Acacia seyalroot was also separated in aqueous, n-hexane and ethyl acetate fractions.Based on the results obtained and reported in the article, all fractions of Acacia seyal root hydromethanolic crudeextract and the fractions separated in aqueous, n-hexane, ethyl acetate fractions, showed anti-diarrheal activity indose-dependent manner. The single component detection in all fractions and crude extract showed the presence ofsimilar metabolites containing steroids, phenols, anthraquinones, and glycosides. However, the specific levels orconcentrations of those metabolites were not detected specifically in each fraction. Therefore, we recommend thedetection of specific levels of each secondary metabolite in each fraction in future studies. It was also unclear whetherthose metabolites are dependent of each other or could be utilized as anti-diarrheal agent in single purified metabolite.The study model used in this study was diarrheal model induced by oil-castor. This study model is a traditional modelwhich had been used in studies for decades. However, under the physiological condition, diarrhea is frequently caused bythe infections. 5 Therefore, the bacteria infection model could be taken as consideration for being used for the follow-upof this study in the near future.6 Overall, this study still convey the novel idea of exploring the anti-diarrheal activitypossessed by the root extract of Acacia seyal which hopefully could be further explored and studied for addressing thesuggestions provided above.AcknowledgmentsWe would like to appreciate all supports given during the study performed by Mengesha, A. K. et al, and their articlepreparation. In addition, appreciations should be given to Dr Sunarno for his continuous support for the authors of thisletter to the editor.Clinical Pharmacology: Advances and Applications 2022:14 111–112 111© 2022 Panjaitan et al. This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessingthe work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).Clinical Pharmacology: Advances and Applications Dovepressopen access to scientific and medical researchOpen Access Full Text ArticleReceived: 18 November 2022Accepted: 25 November 2022Published: 29 November 2022