A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates

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Gu, Xiaoqian and Fu, Liping and Wang, Zhiyan and Cao, Zhe and Zhao, Luying and Seswita-Zilda, Dewi and Zhang, Ao and Zhang, Qian and Li, Jiang (2024) A Novel Bifunctional Alginate Lyase and Antioxidant Activity of the Enzymatic Hydrolysates. Journal of Agricultural and Food Chemistry, 72 (8). pp. 4116-4126. ISSN 0021-8561

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Official URL: https://doi.org/10.1021/acs.jafc.3c08638

Abstract

Alginate lyaseAly448, apotential newmemberof thepolysaccharide lyase(PL)7 family,whichwas clonedand identified fromthemacroalgae-associatedbacterialmetagenomic library, showedbifunctionality.Themolecular docking results revealedthatAly448hastwocompletelydifferentbindingsitesforalginate(polyMG),poly-α-L-guluronicacid(polyG),andpoly-β D-mannuronic acid (polyM) substrates, respectively, whichmight be themolecular basis for the enzyme’s bifunctionality. TruncationalresultsconfirmedthatpredictedkeyresiduesaffectedthebifunctionalityofAly448,butdidnotwhollyexplain.Besides, Aly448presentedexcellentbiochemicalcharacteristics, suchashigherthermal stabilityandpHtolerance.DegradationofpolyMG, polyM,andpolyGsubstratesbyAly448producedtetrasaccharide(DP4),disaccharide(DP2),andgalactose(DP1),whichexhibited excellentantioxidantactivity.Thesefindingsprovidenovel insightsintothesubstraterecognitionmechanismofbifunctionalalginate lyasesandpaveanewpathfor theexploitationofnatural antioxidantagents.

Item Type:	Article
Uncontrolled Keywords:	bifunctionalalginate lyase, alginateoligosaccharides, antioxidantactivity
Subjects:	Medicine & Biology
Depositing User:	Mrs Titi Herawati
Date Deposited:	05 Dec 2025 01:07
Last Modified:	05 Dec 2025 01:07
URI:	https://karya.brin.go.id/id/eprint/55687

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