A New Approach to Select Doubled Haploid Rice Lines under Salinity Stress Using Indirect Selection Index

This study determined the indirect selection index of doubled haploid (DH) rice using a
multivariate analysis approach to select lines adaptive to salinity stress, comprising three experiments.
The first experiment involved the selection of good agronomic characters in a field experiment conducted
at an experimental station in Bogor, Indonesia. The second experiment involved salinity tolerance
screening through hydroponic cultures using 0 and 120 mmol/L NaCl, conducted at a greenhouse in
Bogor. The third experiment involved the validation of the indirect adaptability selection index (IASI)
through a field experiment in Sukra (saline area). Field experiments followed a randomized complete
block design (RCBD), whereas an RCBD nested factorial design was used for the greenhouse
experiment. The first and second experiments used 56 DH lines and four check varieties with three
replications. In the second experiment, Pokkali and IR29 varieties were also added as tolerant and
sensitive checks of salinity, respectively. The third experiment used 28 selected DH lines, Inpari 29 and
one sensitive DH line. The good agronomic index (GAI) was 0.465 yield + 0.433 number of productive
tillers + 0.31 number of filled grains. This generated 24 DH rice lines with good agronomic traits. The
salinity tolerance index (SaTI) was developed through the average of standardized salinity tolerance
score and salinity selection index based on discriminant analysis. This generated 34 DH rice lines with
good salinity stress tolerance. The IASI (IASI = GA
SaTI) selected 28 DH rice lines adaptive to salinity
stress and it was considered effective by Sukra validation.