Utilization of Leiotrametes menziesii BRB 73 for Decolorization of Commercial Direct Dyes Mixture with Different Culture Conditions

Mycoremediation is classified as an inexpensive, environmentally friendly, and effectivetechnique to reduce wastewater. Leiotrametes menziesii BRB 73 was one of the White RotFungi (WRF) that has the potential to degrade dyes. Suitable environmental conditions canoptimize dye decolorization results. This study aims to investigate optimal environmentalconditions such as time incubation, concentration of dyes, pH, CuSO4, and glucoseconcentration against decolorization of a mixture of direct dyes and enzyme activity (laccaseand MnP). The mixture of commercial direct dyes used contains direct turquoise (DT),direct orange (DO), and direct yellow (DY) dyes. Decolorization was measured using aspectrophotometer at 400-700 nm. Laccase and MnP assay using ABTS and 2.6 DMP assubstrate, respectively. The highest decolorization by Leiotrametes menziesii BRB 73 wasproduced at 54.3% at 96 hours and increased to 67% at a dye concentration of 500 mg.L-1.Meanwhile, the highest laccase and MnP activities were 215 U.L-1 and 39 U.L-1, respectively.pH range was quite wide, ranging from pH 5.5-9, supported by stable MnP activity from pH3-7. CuSO4 inducers were not required for the decolorization of these dyes. Decolorizationwas optimal at the addition of 1% glucose, while enzyme activities were 0.5% glucose.Decolorization of dyes by Leiotrametes menziesii BRB 73 was indicated through degradationpathways involving laccase and MnP enzymes. This isolate has a high tolerance to dyeconcentrations, a wide pH range, and low carbon requirements. Thus, it was recommendedas a mycoremediation agent.