Yeast Probiotic Isolated from Fish Fermented (Budu) with Promising AFB1 Biodetoxify

Yeast probiotics which adsorb molecules into the cell walls of microorganisms have the benefit of degrading andpreventing the negative effects of mycotoxin toxicity, especially aflatoxin B1 (AFB1). The research used five types ofprobiotic yeast (Pichia kudriavzevii strain B-5P, Pichia kudriavzevii strain CBS 5147, Saccharomyces cerevisiae (SC1),Saccharomyces cerevisiae (SC2), and Saccharomyces cerevisiae (SC3) to degrade aflatoxin B1 (AFB1). The qualitativeselection used coumarin as a carbon source and using supernatants and non-viable cells and then tested their ability todegrade AFB1. The second selection was carried out quantitatively using HPLC to obtain the final result and find thebest yeast probiotic for degrading AFB1 in vitro. The research results showed that five yeast probiotics could degradeAFB1 using coumarin as a carbon source. The next selection was to use supernatant and non-viable cells in degradingAFB1. The non-viable cells provide the highest degradation for P. kudriavzevii strain B-5P, P. kudriavzevii strain CBS5147 and S. cerevisiae (SC1). Both supernatant and viable cells were almost the same in their ability to degrade AFB1in S. cerevisiae (SC2), on the contrary, S. cerevisiae (SC3) had a higher ability in supernatant than viable cells.Gastrointestinal quantitative selection obtained that P. kudriavzevii CBS 5147 has the highest degradation of AFB1 was69.35%. Meanwhile, P. kudriavzevii B-5P has the highest qualitative degradation of AFB1 (78.83%) and quantitativedegraded AFB1 in the digestive tract including isolates treatments non-viable (81.69%), viable (89.26%), andsupernatant (77.54%). Based on the research results, P. kudriavzevii B-5P is an attractive candidate to be selected forthe biological detoxification (biodetoxification) of AFB1 in vitro.