Sponge molecular screening for antimicrobial genes by PCR

Rosmiati, Rosmiati and Emma, Suryati and Andi, Parenrengi and Sulaeman, Sulaeman (2007) Sponge molecular screening for antimicrobial genes by PCR. Indonesian Aquaculture Journal (IAJ), 2 (2): 7. pp. 127-131. ISSN 0215-0883

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Abstract

Molecular biotechnology approach has been applied on sponge for preventing diseases on fishery culture. This is important for anticipating and avoiding the using of amount of sponge in nature. The present study aims to screen the antimicrobial (oxytetracycline and chloramphenicol) genes of sponge. DNA extraction of samples was done using the DNeasy Plant mini kit, Phenol-Chloroform and modification of Phenol-Chloroform methods. The presence of oxytetracycline and chloramphenicol genes in sponge was detected using Polymerase chain reaction (PCR) technique. Result of the study showed that four species (Sylotella aurantium, Acanthella kletra, Gelliodes fibulatus and Auletta sp. were amplified for oxytetracycline and two species (Auletta sp. and Pericharax sp.) of sponge were amplified for chloramphenicol at each 226 bp.

Item Type: Article
Additional Information: Validated by Sri Wulan
Uncontrolled Keywords: Sponges, Chloramphenicol, Oxytetracycline
Subjects: Medicine & Biology > Cytology, Genetics, & Molecular Biology
Agriculture & Food > Fisheries & Aquaculture
Depositing User: - Elfrida Meryance Saragih
Date Deposited: 19 Jun 2024 03:44
Last Modified: 19 Jun 2024 03:44
URI: https://karya.brin.go.id/id/eprint/37563

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