Automated Detection of Congested Central Vein Liver Histology of Mice Infected with Plasmodium berghei Using CellProfiler 2.0

Malaria is initiated by Plasmodium sporozoites
infections, which are inoculated by mosquitoes.
Histopathologic lesions often described in the liver of
rodent with malaria are congested central vein with
neutrophils and eusinophils within the lumen. Detection of
congested central vein has a possibility to do automatically
using image analysis software. Here the used of
CellProfiler an open access cell image analysis software for
automated detection congested central vein liver histology
of mice infected with Plasmodium berghei is reported. The
results are compared to the manual detection. Wilcoxon
rank test was used for statistical analysis with H0
hypothesis that means there was no significant difference
between manual analysis and those with CellProfiler.
Totally 10 images were analysed for both manually and
using CellProfiler. Results showed that there were no
significant difference between manual and automatic
counting (p>0,05). Overall it appears that in our research
analyzes with CellProfiler are comparable but not better
than manual.