Mutations of the p53 Gene in Nasal NK/T-Cell Lymphoma

Ling, Ti and Mukh, Syaifudin (2000) Mutations of the p53 Gene in Nasal NK/T-Cell Lymphoma. Laboratory Investigation, 80. pp. 493-499.

[thumbnail of Mutation of p53 lab invest 2000.pdf]
Preview
Text
Mutation of p53 lab invest 2000.pdf

Download (1MB) | Preview

Abstract

Mutations of the p53 tumor suppressor gene are reported in various kinds of malignancies including lymphomas. However, p53 gene mutations in nasal NK/T-cell lymphoma have not been reported because most parts of tumors are necrotic
and a small amount of living tumor tissues is available for the molecular study. Expression and mutations of the p53 gene were examined in the paraffin-embedded specimens of the nasal lesions from 42 Chinese (Beijing and Chengdu) and Japanese (Okinawa and Osaka) patients with nasal NK/T-cell lymphoma by the immunohistochemistry and single strand conformation polymorphism (SSCP) analysis of polymerase chain reaction (PCR) amplified products followed by direct sequencing. Thirty single-nucleotide substitution mutations were observed in 20 of 42 cases (47.6%). Among the 30 mutations, 18 were missense (mainly G:C to A:T transitions), 9 were silent, and 1 was a nonsense mutation. The remaining 2 mutations involved intron 5 and
exon 5 terminal points. Abnormal expression of the p53 protein was also observed in 19 of 42 (45.2%) cases. The incidence was significantly (4-fold) higher in the cases of Osaka than those in other areas, although the incidence of p53 mutations in the cases of Osaka was one-half to one-third of those in the other three areas. The results may suggest some racial, environmental, or lifestyle differences in the cause of nasal tumorigenesis

Item Type: Article
Subjects: Taksonomi BATAN > Keselamatan dan Keamanan Nuklir > Keselamatan Radiasi dan Kesehatan kerja > Keselamatan dan Kesehatan Kerja
Taksonomi BATAN > Keselamatan dan Keamanan Nuklir > Keselamatan Radiasi dan Kesehatan kerja > Keselamatan dan Kesehatan Kerja
Divisions: BATAN > Pusat Teknologi Keselamatan dan Metrologi Radiasi
IPTEK > BATAN > Pusat Teknologi Keselamatan dan Metrologi Radiasi
Depositing User: Administrator Repository
Date Deposited: 26 May 2018 05:54
Last Modified: 31 May 2022 04:03
URI: https://karya.brin.go.id/id/eprint/2380

Actions (login required)

View Item
View Item