Lipopolysahcarids injected on haemolypmh of redclaw (Cherax quadricarinatus): a study in the immune system

The aim of this study was to study about LPS induction from Aeromonas hydrophyla, to improve innate immunity of red claw (Cherax quadricarinatus). There were any test at the hemolymph had been performed to reach the direction, that were activation of prophenoloxidase (proPO) and fagocytosis activity. Material used was Red claw 40 species. The method used was experimental method with Complete Randomized Design consisted of 4 kinds of treatment: P1 (without injection of LPS), P2 (LPS: 0.5 mg/ml), P3 (LPS: 1.0 mg/ml), and P4 (LPS: 1.5 mg/ml). Each treatment was performed triplicate. Replication performed was the taking of hemolymph sample at 2 nd and 3rd d and 3rd week after injection of LPS. Research parameters evaluated were activation of prophenoloxidase (proPO), phagocytosis activity, and gene characterization responsible at the immune system. Data, was analyzed by Analysis of Varians, and was continued by BNT test. It could be conclude that induction of LPS by injection at red claw species was not significantly difference (P>0.05) against activation of proPO, was significantly difference with phagocytosis activity (P<0.05). injection of LPS at 1 mg/ml concentration or 1.5 mg/ml showed the highest phagocytosis activity.