Trisindoline 5 compound inhibits human breast cancer stem cell formation by downregulating the BCL-2 gene expression

Background: Breast cancer cells growth and survival are supported by breast cancer stem cells (bCSCs) mammosphere formation. bCSCs represent a subpopulation of undifferentiated cancer cells which associated with self-renewing abilities, tumor initiation, drug resistance and metastasis. Overexpression of B Cell Lymphoma 2 (BCL-2) family in many tumor cells contributes to apoptosis resistance. Trisindoline is an indole trimer alkaloid natural compound that provide a cytotoxic effect on cancer cells. A new modification of trisindoline has been synthesized into trisindoline 5 in 2021. Objective: This study purposed to investigate the effect of trisindoline 5 compound against BCL-2 gene expression in bCSCs in vitro. Methods: The bCSCs MDA-MB-231 were divided into control and treatment groups which futher analysed in gene expression using qPCR Livak method. Results: Based on gene expression analysis, the results showed that trisindoline 5 may decrease the expression of BCL-2 in MDA-MB-231 cells. Conclusion: This study concludes that trisindoline 5 could downregulate the antiapoptotic BCL-2 gene expression in bCSCs in vitro.