Fractionation and characterization of semi polar and polar compounds from leaf extract nicotiana tabaccum L. reflux ethanol extraction results

The decline in cigarette production as the solution of health problems can interfere with the welfare of tobacco
farmers in Indonesia. So, it is required to utilize the alternative uses of tobacco with chemical compounds inside it as the raw material for producing alternative products. One of the methods that is efficient in separating chemical compounds
from plant extracts is fractionation and characterization method. This method has never been used for Nicotiana
tabaccum L. extract using semi polar and polar solvents. This study begins with preparing Nicotiana tabaccum L. extract ingredients obtained through reflux ethanol extraction process. Extracts are analyzed by HPLC which serves to determine the chemical compounds in tobacco extract qualitatively. Extract that has been analyzed, is then fractionated using column chromatography with semi polar (ethyl acetate) and polar (ethane) solvents sequentially. Chemical compounds from tobacco extracts will be dissolved in accordance with the polarity of each solvents. The chemical compound is then characterized using HPLC quantitatively and qualitatively. Then, the data that has been obtained is used to find the partition coefficient of the main components in Nicotiana tabaccum L., which is Nicotine (kN) in Virginia 1 (Ethyl Acetate) fraction at 0.075; Virginia 2 (Ethyl Acetate) fraction at 0.037; And Virginia 3 (Ethyl Acetate) fraction at 0.043