An Alternative simple method for preparing and preserving cross-section of leaves and roots in herbaceous plants: case study in Orchidaceae

This alternative method provides a simple and faster procedure for preparing cross-sections of leaves and roots in herbaceous plants, especially for living specimens of orchids (Orchidaceae). This method used a clamp-on hand sliding microtome to make cross-sections of leaves and roots, with sections preserved inside the microtubes containing preservation liquid. This preservation technique allowed the sections to be restained and to be used for further usage in future. This method was more practical than the paraffin embedding method because it does not need theadditional steps of paraffin embedding and deparaffinization. It may also provide better cross-section results than free-hand sectioning method. The procedure is very feasible and is recommended for use in plant anatomy observation.