Pengaruh prototipe enkapsulasi berbasis alginat terhadap viabilitas dan stabilitas sel punca mesenkim

Research in the field of stem cells is currently growing very quickly. Hospitals in Indonesia have used stem cells
as an alternative to cure some diseases such as diabetes, heart disease, fractures and joints, dental implants,
and others. In Indonesia alone there are 11 hospitals that have been using treatment with stem cells such as
RSCM and RS. Soetomo as a pain rmah Pembina. Today, adult stem cells can be obtained not only from the
spinal cord and peripheral vessels, but also from human body fatty tissue, which can be isolated as adherent
stem cells (mesenchymal stem cells). The consideration of fatty tissue as a source of mesenchymal stem cells
(MSCs) for autologous tissue engineering is that they are available in abundant quantities through minimally
invasive procedures, as well as are easily cultured and reproduced. It is possible to breed and differentiate in
the desired direction of the tissue. However, stem cell growth requires special conditions to grow such as
requiring optimum growth conditions such as ambient temperature of 37 ° C and a concentration of 5% CO 2.
Maintenance MSC also requires a subculture process, that is the process of moving MSC from full culture
media to new media, continuous subculture process can cause changes in MSC. The survival of stem cells may
be disrupted by micro-conditions such as hypoxia, oxidative stress. Cell encapsulation is a method of cell
capture in semipermeable polymer membranes, generally biocompatible encapsulation materials such as
chitosan, hyaluronic acid, and alginate. The ability of alginate to form 3D structure and low toxicity
characteristic and high biocompatibility make alginate widely used for encapsulation process. Encapsulation is
generally done to protect cells. Therefore, the purpose of this study was to determine whether alginate-based
encapsulation can improve stability viability and stability by using different concentrations of alginate and
CaCl2.