Karakteristik fermentasi dan kecernaan in vitro senyawa bioaktif ampas teh (Camellia sinensis) sebagai protein bypass dan daun kembang sepatu (Hibiscus rosa-sinensis L) = In vitro fermentability and digestibility characteristic of feed containing bioactive compound of tea by product (Camellia sinensis) and (Hibiscus rosa-sinensis L) leaves as bypass protein

Tea by product (Camellia sinensis) is a source of tannin acid utilized to protect feed protein from excessive degradation of rumen microbes. Leaf hibiscus (Hibiscus rosa-sinensis L) containing saponin bioactive compounds is a partial defaunasi agent to reduce the population of protozoa in the rumen. The objective of this experiment was designed to obtain an optimum dose combination of tea by product (Camellia sinensis) and Hibiscus rosa-sinensis L leaf based on in vitro fermentability and digestibility characteristic. The control diet was elephant grass (Pennisetum purpureum Schumacher) and concentrate with 60:40 % DM ratio. Factorial randomized block was used, consisted tea by product (0; 1; 2; 3 mg/ml fluid) and Hibiscus rosa-sinensis L leaf (0; 0.15; 0.3 mg/ml fluid). Variables observed are total VFA, N-NH3, pH value, dry matter and organic digestibility. The results showed that there were no interaction effect between two factors on parameters observed. The addition of 1-3 mg/ml tea by product decreased (P<0.05) N-NH3 concentration, dry matter and organic matter digestibility but did not affect pH value. The addition of 0.15-0.3 mg/ml Hibiscus rosa-sinensis L leaf did not affect N-NH3 concentration, pH value, dry matter and organic matter digestibility. The addition of 1 mg/ml tea by product or 0.3 mg/ml Hibiscus rosa-sinensis L leaf increased (P<0.05) total VFA production.