Solvent Extraction Research and Development, Japan
Online ISSN : 2188-4765
Print ISSN : 1341-7215
Original Articles
Extraction of Resveratrol from Melinjo (Gnetum gnemon L.) Seeds Using Mixtures of Liquid Carbon Dioxide and Ethanol
Kenji MISHIMA, Yusraini Dian Inayati SIREGAR, Hirofumi KAWAMURA, Ryo KAWAKAMI, Shota ITO, Yuuta INOUE, Tetsuya HIROTA, Takunori HARADA, Takafumi KATO, Makoto MISUMI, Tadashi SUETSUGU, Keiichi IRIE, Kenichi MISHIMA, Salim MUSTOFA, Fauziyah HASANAH, Hilyatuz ZAHARO, Lily Surayya Eka PUTRI, Agus SALIM
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2015 年 22 巻 1 号 p. 69-77

DOI https://doi.org/10.15261/serdj.22.69
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The extraction of resveratrol from Melinjo seeds (Gnetum gnemon L.) using mixtures of liquid carbon dioxide (CO2) and ethanol was performed at 5, 20 and 25 ºC. The extraction pressure was from 8 to 14 MPa. The extraction yields were compared with the yields obtained by other solvent extraction methods, such as pure liquid CO2 extraction and conventional aqueous alcohol (methanol or ethanol) extraction. We conducted qualitative and quantitative analyses for resveratrol in the extract using HPLC and analyzed the extraction behavior. The effect of three operating parameters, temperature, pressure and composition of ethanol in liquid CO2, on the extraction yield was investigated using the single-factor method. The yields of resveratrol in the extraction were significantly improved by adjusting the operating temperature and the amount of ethanol in liquid CO2.

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© 2015 Japan Association of Solvent Extraction
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