Heritability, Selection Response, Genetic Gain and RAPD Markers of Anabas testudineus in Three Generations

The acceleration of fish growth is often accomplished through individual selection programs.Therefore, this study aimed to evaluate growth performance in climbing perch (Anabas testudineus) over three generations by analyzing heritability, selection response, as well as genetic gain and distance using the molecular marker RAPD.Climbing perch broodstock was sourced from Musi River in South Sumatra, which produced three individually selected offspring.A total of three treatment types were used, namely first-generation (F1), second-generation (F2), and thirdgeneration (F3).The treatment was replicated four times, and the experiment utilized a randomized design.Furthermore, gene diversity was compared among generations and natural populations using 19 primary RAPD markers.The results showed that low and high heritability was found in F1 (0.18) and F3 (0.40), respectively.The lowest selection response was observed in F1 (3.09g), while F3 (8.04g) demonstrated the highest.F1 also had the smallest genetic gain (5.58%), and F3 produced the largest (13%).Genetic distance (0.28) between F3 and the wil d population was not significantly different.The results indicated that the next generation of climbing perch might not experience a significant genetic increase.