Chloroplast Genome of Myristica fragrans : Assembly, Annotation, and Phylogenetic Relationships

Myristica fragrans is a native and economically significant species in indonesia. However, itscomplete chloroplast genome, particularly from indonesia, has not been previously reported.in this study, we successfully sequenced and assembled the complete chloroplast genome ofM. fragrans using next-generation sequencing technology. the genome has a total length of155,868 bp and exhibits a typical quadripartite structure, consisting of a large single-copy(LSC) region (87,062 bp), a small single-copy (SSC) region (20,664 bp), and a pair of invertedrepeats (iRs) (24,071 bp each). Genome annotation identified 126 genes, including 83protein-coding genes, 8 ribosomal Rna genes, and 35 transfer Rna genes. a total of 141simple sequence repeats (SSRs) were detected, with mononucleotide repeats being the mostprevalent (90.07%). phylogenetic analysis using six Myristica chloroplast genomes and threeoutgroup species confirmed that M. fragrans is most closely related to M. argentea, providingnew insights into the evolutionary relationships within the Myristicaceae family