Characterization and Bioactivities of Sequentially-Prepared Sea Cucumber Ethanolic Extracts and Protein Hydrolysates

The sea cucumber ethanol extracts (SCEEs) and protein hydrolysates (SCPHs)of Holothuria atra(HA), H. leucospilota(HL), and Bohadschia marmorata (BM)were prepared sequentially using 60% ethanol and Neutrase. The SCEE of HLcontained phenolic acids (4.63 ± 0.50 µg GAE/mg extract) and was the mostpotent radical scavenger (91.76 ± 0.76%) at 10 mg/mL; whereas its SCPHcontained hydrophobic amino acids (31.25 g/100 g) and inhibited glycationby 86.16 ± 5.92% at 5 mg/mL. Furthermore, the SCEE of HA inhibited tyrosi-nase by 49.93 ± 0.27% at 10 mg/mL, related to sulfated saponins (121.82 ±3.52 µg xylose/mg extract). This study reports SCEEs as antityrosinase andSCPHs as antiglycation agents for the first time.