CHANGES IN THE PROFILE OF VOLATILE COMPOUND IN SEED STORAGE OF RUBUS FRAXINIFOLIUS

Storage conditions are one of the factors that affect seed quality. However, storage cannot improve seed quality, but it can maintain seeds in good condition, including their volatile compounds. Therefore, the study aimed to determine the changes of volatile compounds during seed storage on Rubus fraxinifolius seeds. The seeds were obtained from Cibodas Botanical Garden and divided into three groups i.e., seed without storage, seed stored at room temperature for four months, and seed stored in the refrigerator for four months. The seed extract using ethanol and methanol solvents was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). The results showed that the total volatile compounds detected in the ethanol and methanol extracts of R. fraxinifolius seed were 113 and 80, respectively. Seven types of main volatile compounds are found in all seed treatments and solvents included g-Sitosterol, g-Tocopherol, 9-Octadecenamide, (Z)-, 9,12-Octadecadienoic acid (Z,Z)-, 9,19-Cyclolanostan-3-ol, 24-methylene-,(3.beta.)-, dl-a-Tocopherol, and Squalene. Compounds were belong to sterols, vitamin E, fatty acids, and triterpenoids. In addition, seed storage treatment increased the percentage of specific volatile compounds, such as g-Sitosterol, g-Tocopherol, 2,4-Decadienal, (E,E)-, 2,4-Decadienal, (E,Z)-, 9-Octadecenamide, (Z)-, 9,12-Octadecadienoic acid (Z,Z)-, 9,19-Cyclolanostan-3-ol,24-methylene-, (3.beta.)-, dl-a-Tocopherol, Eicosane, n-Hexadecanoic acid, and Squalene.