Kadar Karbohidrat dan Penapisan Senyawa Aktif dari Pleurotus pulmonarius yang Diekstraksi Menggunakan Metode Microwave Assisted Extraction (MAE) dengan Beberapa Perlakuan Suhu

The majority of carbohydrates found in edible mushroom are in the form of polysaccharides, specifically beta glucans. These beta-glucans have been reported as anticancer, antioxidant, and immunomodulator. This study aimed to investigate the potential of P. pulmonarius as functional food by comparing the carbohidrate content (total carbohydrate, beta-glucans, and reducing sugars) in P. pulmonarius extracted using microwave assisted extraction (MAE) at different temperatures: 600C, 800C and 100 0C. Moreover, active compound screening was also conducted. MAE was chosen as the extraction method due to its short extraction time. The phenol-sulfiric acid method was used to measure total carbohydrates, while beta-glucan content was measured using the Megazyme Beta-Glucan Kit. Reducing sugar content was measured using the DNS (3,5-Di Nitro Salisilic Acid) and active compound screening was performed by qualitative method. The results showed that the highest total carbohydrates and beta-glucans were obtained at 60 0C (75,089±0,012 mg/mL and 32,356±0,006% w/w respectively) while the highest reducing sugar content was obtained at 80 0C (0,216±0,009 mg/mL). Active compound screening revealed the presence of flavonoids, saponins, and triterpenoids in each sample. These findings suggest that P. pulmonarius has potential as functional food since the rich content of beta-glucans and active compounds. The use of MAE at 60 0C is more effective to extract P. pulmonarius compared with 80 0C and 100 0C.