ANTI-DIABETIC EFFECT OF Anredera cordifolia (Ten.) Steenis (BINAHONG) ON DIABETES GENE MARKERS EXPRESSION

The evidence of molecular mechanism of Anredera cordifolia (Ten.) Stennis (binahong) as an anti-diabetic-has not been elucidated. In this study, we investigated the potential of 70% ethanolic extract of binahong as antidiabetic by evaluating its effect on two diabetic gene markers expression, including Glucose Transporter 4-(GLUT4) and Peroxisome Proliferator-Activated Receptor Gamma (PPAR-γ) in 3T3-L1 adipocytes. The investigation has been carried out by semi quantitative real time PCR. Our finding showed the GLUT4 mRNA-levels were dose dependent excess in adipocyte which was treated by 6.25-12.5 μg/ml of 70% ethanolic extract. However, the GLUT4 expression level went down to 1.2-fold at 50 μg/ml dose. In addition, the pattern of PPARγ mRNA levels in 3T3-L1 were almost the same. Every extract produced an increment on PPAR-γ mRNA levels-except at 3.125, 6.25, and 50 μg/ml doses. In undifferentiated adipocytes, GLUT4 and PPAR-γ mRNA were-barely expressed. We assumed that the up regulation of GLUT4 and PPAR-γ mRNA levels on adipocytes due to-the effect of binahong active compounds. However, the exact compounds have not been discovered yet. We-hypothesize that these compounds act as a ligand for PPAR-γ transcriptional factor supporting the expresion of-the GLUT4 in glucose homeostasis at molecular level.