Antioxidants, Total Phenolic and Flavonoid Content and Toxicity Assay of Ampelas (Tetracera macrophylla Wall.Ex Hook.F.& Thoms) From Kalimantan-Indonesia

Background: High Reactive Oxygen Species (ROS) contribute to disease pathogenesis. Phenolic compounds and flavonoids are effective as antioxidants.

Objective: This research aimed to measure the antioxidant activity, total phenolic and flavonoid content and leaf toxicity of Tetracera macrophylla.

Methods: DPPH and FRAP were used to determine antioxidants, and the Folin–Ciocalteu method was used for total phenolic content, total flavonoid content with AlCl3 and toxicity with MTT assay against RAW 264.7 cells.

Results: Methanol extract has antioxidant activity with IC50 = 81.582 μg/mL (DPPH) and 11840 mol/g (FRAP), total phenolic content of 353.781 mg GAE/g dry weight, and flavonoid content of 279.2 mg QE/g dry weight. The ethyl acetate and n-hexane extracts had weaker antioxidant activity than the methanol extracts. The IC50 toxicity assay methanol extract and ethyl acetate extract respectively showed 288.792 μg/mL and 541.472 μg/mL.

Conclusion: The methanol extract of Tetracera macrophylla showed the highest yield, total phenolic content and total flavonoid content and had the highest antioxidant activity. Methanol extract has low toxicity to RAW 264.7 cells.