Allele frequencies and data from 23 STR loci in the Tengger population, Indonesia

Introduction: Social interactions such as marriage patterns in communities descended from the Tengger ethnic group are hypothesized to affect genetic variations that occur in communities descended from the Tengger ethnic group in the Tengger highland area. DNA identification using Short Tandem Repeat (STR) is a reference for assessing genetic variations that exist in populations. This research aims to determine allele frequencies and genetic variations in the Tengger tribe in East Java, Indonesia. Method: This observational study used 50 from the Tengger tribe. DNA isolation was done using the Promega kit, PCR was done using 23 autosomal STR loci, and visualization was done using Capillary electrophoresis to obtain allele data from all loci. The data is analyzed and then presented using tables and graphs. Result: The results show various allele frequencies and the D10S1248 locus on allele 13 has the highest frequency with 0.5300. The percentage of homozygous and heterozygous alleles shows that the CSF1PO locus has a homozygous allele percentage of 42% and the D21S11 and SE33 loci have a heterozygous allele percentage of 94%. The highest Heterozygosity value is found at the SE33 locus with a value of 0.9098 and the highest Homozygosity value is at the CSF1PO locus with a value of 0.3864. Conclusion: The various allele frequencies and forensic data obtained conclude that the Tengger tribe has unique characteristics.