Avicularin: The major secondary metabolite isolated from Saurauia vulcani as a potential anti-colorectal cancer agent

Saurauia vulcani flora develops certainly in North Sumatra Province, Indonesia. It is normally consumed as an
antidiabetic remedy and a treatment related to digestion diseases. This research aims to isolate the major compound
from S. vulcani plants and assess the bioactivity of the S. vulcani plant for its anti-colorectal cancer potential.
Subsequently, the ethyl acetate fraction was analyzed with a solvent system used as the eluent ethyl acetate:
methanol: Aquadest = 4.6:0.2:0.2. Utilizing spectroscopy, we determined the chemical structure of the remote
substance. Utilizing the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide technique in vitro, the
anti-colorectal cancer activity was demonstrated against WiDr cancer cells. This study revealed that the major
compound was identified as avicularin. The avicularin compound is a flavonoid group known to have anticancer
activity. The cytotoxicity of avicularin compound was assessed against the WiDr cell line, with a 50% inhibition
concentration value of 521.14 μg/ml. The bioactivity of the ethyl acetate fraction is stronger than that of the isolated
part (avicularin compound).