STERILITY OF MALE AEDES AEGYPTI POST γ - RAY STERILIZATION

Aedes aegypti (Ae. aegypti ), avector of dengue hemorrhagic fever (DHF), population control was developed using the sterile insect technique (SIT). Sterilization process was conducted using γ-ray sterilization. To support the SIT application, the sterility of male Ae. aegypti on the 1st-5th days post sterilization were studied. The first step of this research was the sterilization process of male Ae.aegypti with 70Gy of γ-ray sterilization. Then, the sterile male Ae.aegypti mosquitoes on the 1st ; 2nd ; 3rd; 4th and 5th days after the sterilization process espectively were competed with un-irradiated males to mate with un-irradiated females. The number of eggs that were produced was incubated to determine the fertility of sterile male Ae. aegypti. The result showed that the average number of eggs produced from mating combination of sterile male Ae.aegypti mosquitoes on the 1st-5th days post sterilization, un-irradiated females and un-irradiated males
was 1,135; 1,118; 1,243, 1,372 and 1,326.The average
percentage number of eggs that did not hatch into larvae
was 97.69%; 97.87%;90.92%, 96.20%,
and
86.91%.
By
analysis
of
variance
(ANOVA),
the
number
and
percentage
of
the
unhatched
eggs
were
not
significantly
different
at
the
level
confident
of
95%.
The
research
showed
that
the
mating
ability
and
sterility
of
sterile
male
Ae.
aegypti
on
the
1
st
-
5
th
days
post
γ
-
ray
sterilization
show
no
significant
difference