PREPARASI DAN STUDI AWAL BIODISTRIBUSI 99mTc -IMUNOGLOBULIN M YANG AKAN DIGUNAKAN SEBAGAI PREPARAT PENATAH INFEKSI/INFLAMASI

Sari

PREPARATION AND BIODISTRIBUTION STUDY OF 99mTc . IMMUNOGLOBULIN M AS INFECTION/INFLAMMA TION IMAGING AGENT. The
superiority of radiopharmaceutical compare to the other techniques of medical services, especially for diagnosis and therapy of several deadly diseases such as cancer or diagnosis of infection and iflammmation . Infection diseases are common in Indonesia, Nuclear medicine techniques which uses polyclonal antibody based radiopharmaceutical labeled with technetium-99m offers an alternative method of diagnosis infection/inflammation. Preparation of 99mTc- Immunoglobulin
M(99mTc_IgM) and its analysis have been carried out. This preparation needs several steps, first reducing IgM using mercaptoetanol with molar ratio 1:2000-12000, purification using PO-IO column ( sephadex G-25,Pharmacia), and the reduced IgM was labeled with 99mTcand MDP as
transchelator. The reduced IgM was analysed using size exlusion_HPLC The radiochemical purity of 99mTc_IgM was analysed using TLC/paper chromatography. The stability in the human body was carried out by using fresh human serum after 1 and 2 hours incubation, Biodistribution test on
infected mice was carried out, the radiochemical purity of 99mTc_IgM analysed with TLC/paper chromatography was higher than 90 % for molar ratio Ab:Me 1:12000. The stability of labeled IgM in fresh human serum was stable after 1 and 2 hours incubation, biodistribution test showed higher uptake in the site of infection ( right thigh) compare to the site of uninfection ( left thigh).

Keywords: Antibody, immunoglobulin-M, biodistribution,99mTc, infection/inflammation
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