Pembuatan, Karakterisasi dan Uji In Vitro Nanopartikel Emas Berbasis Konjugat Gom Arab-Vinkristin

Ratih Dyah Pertiwi; Joshita Djajadisastra; ABDUL MUTALIB; Anung Pujiyanto

Ratih Dyah Pertiwi UNIVERSITAS INDONESIA
Joshita Djajadisastra UNIVERSITAS INDONESIA
ABDUL MUTALIB UNIVERSITAS PADJADJARAN
Anung Pujiyanto UNIVERSITAS ESA UNGGUL

Abstract

Gold nanoparticles (AuNP) are potentially developed as nanomedicine because AuNP is easily synthesized, functionalized, and biocompatible. With gum arabic as a stabilizer, vincristine was conjugated with gold nanoparticles. As a reducing agent, it used 0.02 M Natrium Boro Hidrat (NaBH₄) solution. Gold nanoparticles (AuNP) coated with conjugated gum Arabic (GA) and vincristine (VCR) were successfully synthesized and characterized. The conjugation of GA-VCR and AuNP displayed a narrow hydrodynamic particle size distribution with average size < 100 nm by TEM and PSA (particle size analyzer). We investigated the cytotoxic activity of conjugated vincristine-gum arabic-gold nanoparticle by tetrazolium salt assay (MTT) using cancer cell line CCR-CEM. Cytotoxic activity of conjugated VCR-GA-AuNP before and after purification by Size Exclusion Chromatography (SEC), against leukemia cell line CCRF-CEM, was described by IC₅₀ value. All formulation had a cytotoxic of activity with IC₅₀ <20 μg/ml. The IC₅₀ of samples against CCRF cell line were 1,026 μg/mL and 2,607 ug/mL, respectively.

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