Humoral immune response induced with dengue virus-like particles serotypes 1 and 4 produced in silkworm

Doddy, Irawan Setyo Utomo and Sabar, Pambudi and Enoch, Y. Park (2022) Humoral immune response induced with dengue virus-like particles serotypes 1 and 4 produced in silkworm. AMB Express, 12 (8). pp. 1-12.

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Dengue is an arboviral disease, which threatens almost half the global population, and has emerged as the most
signifcant of current global public health challenges. In this study, we prepared dengue virus-like particles (DENV�LPs) consisting of Capsid-premembrane-envelope (CprME) and premembrane-envelope (prME) polypeptides from serotype 1 and 4, which were expressed in the silkworms using Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid. 1CprME, 1prME, 4CprME, and 4prME expressed proteins in hemolymph, and the molecular weight of the purifed proteins was 55 kDa, respectively. The purifed polypeptides formed spherical Dengue virus-like particles (DENV-LPs) with ~ 30–55 nm in diameter. The immunoelectron microscopy (IEM) images revealed antigens to the surface of a lipid bilayer of DENV-LPs. The heparin-binding assay shows a positive relationship between absorbance and E protein domain III (EDIII) quantity, which is supported by the isothermal titration calorimetry assay. This indicates a moderate binding afnity between heparin and DENV-LP. The high correlation between patient sera and DENV-LP reactivities revealed that these DENV-LPs shared similar epitopes with the natural dengue virus. IgG elicitation studies in mice have demonstrated that DENV-LPs/CPrMEs elicit a stronger immune response than DENV-LP/prMEs, which lends credence to this claim.

Item Type: Article
Additional Information: DDC'23: 616.92
Uncontrolled Keywords: Dengue virus; Capsid; Premembrane; Envelope, Dengue virus-like particle; Silkworm
Subjects: Medicine & Biology
Divisions: OR Kesehatan > Bahan_Baku_Obat_dan_Obat_Tradisiona
Depositing User: Rasty -
Date Deposited: 15 Dec 2022 03:43
Last Modified: 15 Dec 2022 03:43

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