Sabar, Pambudi and Etik, Mardliyati and Silmi, Rahmani and Damai, Ria Setyawati and Tika, Widayanti and Angelina, Gill and Asri, Sulfianti and Whinie, Lestari (2018) The Potency of aluminum hydroxide nanoparticles for dengue subunit vaccine adjuvant. Microbiology Indonesia, 12 (3). pp. 99-105. ISSN 1978-3477
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Abstract
The potency of aluminum hydroxide as an adjuvant in vaccine development is considered to depend on its particle size. In previous studies, we have successfully prepared two size particle, micro, and nano, aluminum hydroxide gel (alum) adjuvants. The potency of those particles as a candidate of adjuvant is needed to be characterized. In this study, we formulated our adjuvants with purified DENV3 pre Membrane Envelope (prM-E) recombinant protein and evaluated the induction of nitric oxide level in mouse macrophage RAW 264.7 cells. We prepared the alum adjuvant by precipitation-homogenization methods with an agitation rate at 11,000xg. Secreted prM-E recombinant protein was collected from Pichia pastoris X-33 fermentation which produced using bioreactor. Recombinant protein purification was carried out by anion exchange chromatography followed with size exclusion chromatography. The purified prM-E recombinant protein was observed as a single band around 70 -1 kDa with a concentration of 105 mg mL . Complex nanoparticles alum with prM-E protein significantly (p<0.05) induced the nitric oxide level. Further analysis should be conducted in order to discover the detail molecular mechanism of nanoparticle alum adjuvant, recombinant protein, and cellular immune response.
Item Type: | Article |
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Additional Information: | DDC'23: 571.96 |
Uncontrolled Keywords: | Adjuvant, Dengue, Nitric oxide, prM-E |
Subjects: | Medicine & Biology > Immunology |
Divisions: | OR Kesehatan > Vaksin_dan_Obat |
Depositing User: | Rasty - |
Date Deposited: | 10 Nov 2022 03:01 |
Last Modified: | 21 Nov 2022 06:46 |
URI: | https://karya.brin.go.id/id/eprint/12962 |