Ultraviolet to visible spectrophotometry observation to find appropriate wavelength for non-invasive blood glucose level measurement optical device

The wavelength convention for non-invasive blood glucose measurement remains inconclusive. Objective. We have done this experimental observation to find the appropriate wavelength candidate for non-invasive blood glucose level measurement optical device in 190 nm to 900 nm range. We run this observation in Prodia Kedoya and Biochemistry Lab, Universitas Krida Wacana, West Jakarta July 2019. We have obtained fasting, 15 mnt postprandial, and 30 mnt postprandial blood sample from 10 randomly selected consenting non blinded healthy adult subjects between 18 years old to 60 years old. We have diluted each blood samples 400 times in double distilled water before spectrophotometric measurement. We have measured the spectrophotometric absorbance of each blood sample. We then compared it to blood glucose level by standard gold measurement. We have compared the Pearson correlation, and the standard deviation of all samples to then existing wavelength source LED provided by Thorlabs. We have found that the smallest standard deviation is at 510 nm, and the highest Pearson correlation is at 511 nm. There is low UV-VIS absorption value at 490 nm, 505 nm, and 525 nm wavelength. We did not find a severe adverse effect from each participant. Discussion and Conclusion. The 490 nm, 505 nm, and 525 nm wavelength may appropriate for non-invasive blood glucose level measurement optical device. Further research should confirm this trial with human observation.