Combination effect of nutritech feed additive containing saponin, tanin and eugenol essential oils on in vivo rumen methane production in dairy cattle using open circuit respiration chamber technique

Diet manipulation containing feed additive for reducing enteric methane emission of ruminant had been an interesting focus since the GHG’s emission of livestock was reported increasing. Saponin, tannin and eugenol had been selected when they have a good effect for rumen methane gas reduction. There is no more information for combination between saponin, tannin and eugenol which was tested for rumen methane reduction, especially in vivo dairy cattle methane gas production assessment. Therefore, this study was carried out to evaluate the effect of combination of saponin-containing H. Rosa sinensis leaf, tannincontaining tea waste and eugenol-containing clove oil on in vivo rumen methane production assessment in dairy cattle using open circuit respiration chamber technique. The combination was mixed in one feed additive, Nutritech-BPPT. The treatments were R1: control ration (60% forage + 40% concentrate), R2: R1 + nutritech 100 mg/kg of body weight, R3: R1 + nutritech 150 mg/kg of body weight and R4: R1+ nutritech 200 mg/kg of body weight, which were assigned and analyzed in latin square design (4x4). Four dairy cows and four heifers Fries Holland (PFH) were used for in vivo rumen methane production assessment using open respiration chamber technique (MARS-Sable System International). The results indicated that supplementation of nutritech-BPPT reduce methane production from dairy cattle 14.96%, 25.41% and 34.92% after supplementation 200 mg/Kg BW, 100 mg/Kg BW and 150 mg/Kg BW of nutritech, respectively. The combination between saponin, tannin and eugenol essential oil in Nutritech was formulated as an optimum composition after in vitro test and their effect revealed that saponin, tannin and clove oils were no effect on in vitro rumen digestibility. Clove essential oil also may have strong microbial activity from their active component (eugenol). Eugenol is main component of clove oil which its composition was very high more than 90% in clove oil for this study. In conclusion, Nutritech containing saponin, tannin and eugenol essential oil could be used as feed additive for rumen methane reduction in dairy cattle.