Combination Effect of Clove and Orange Peel Oils on in Vitro Digestion of Dairy Total Mixed Ration Using ANKOM DAISYII Incubator

Clove and orange peel oils were used for rumen manipulation in ruminant animal production. However there is limited study with true in vitro rumen digestibility. The objective of this study was to evaluate combination effect of clove and orange peel oils on in vitro digestion of Dairy Total Mixed Ration (TMR) using ANKOM DAISYII Incubator. Ruminal fluid for in vitro digestion technique was prepared as in vitro digestibility ANKOM method. The results indicated that in vitro true DM disappearance (IVTDMD) and in vitro neutral detergent fiber disappearance (IVNDFD) of dairy TMR were significant (P <0.01) affected by clove, orange peel oils and their combination. Clove increased IVTDMD and IVNDFD and energy estimate (TDN, ME and NEl) of TMR, while orange peel oils decreased. Therefore, there was antagonistic effects between CO and OP 300 ppm when they were used together in combination treatment for decreasing in vitro digestion of dairy TMR. 