Biological Evaluation of 99mTc-Kanamycin for Infection Imaging
Keywords:
99mTc-kanamycin, infection, biological evaluation
Abstract
Kanamycin antibiotic was radiolabeled successfully with radioisotope technetium-99m for the potential use as radiopharmaceuticals for infection imaging. 99mTc-kanamycin complexes was prepared 93 % radiochemical purities by direct labelling using 5 mg kanamycin and 30 µg SnCl2. The reaction occurred at alkaline condition (pH=9) and under room temperature for 30 min to achieve high radiochemical purity. Radiochemical purity and stability of 99mTc-kanamycin was determined by ascending paper chromatography using Whatman 3 paper as the stationary phase, and acetone as the mobile phase to separate the radiochemical impurities in the form of 99mTc-pertechnetate. While impurities in the form of 99mTc-reduced were separated using the stationary phase ITLC-SG and 0.5 N NaOH as mobile phase. This study aimed to determine biological characteristic of 99mTc-kanamycin radiopharmaceutical. In vitro cell studies showed that the change of kanamycin structure after labeling with technetium-99m did not give a specific influence to the potency of kanamycin as an antibiotic. In addition on uptake study, a significantly higher uptake of 99mTc-kanamycin with S. aureus than E. coli. Biodistribution of 99mTc-kanamycin complexes was studied on normal and infection mice at 15, 30, 60 and 120 min post-injections. The biodistribution of 99mTc-kanamycin in infection mice showed that the complex accumulated in the infection sites. These results show that 99mTc-Kanamycin radiopharmaceutical have a potential application for infection diagnosis.Downloads
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References
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Widyasari, E. M., Wibawa, T. H. A., & Nuraeni, W. (2013). PHYSICOCHEMICAL CHARACTERISTICS OF THE KANAMYCIN-LYOPHILIZED KIT. Indonesian Journal of Nuclear Science and Technology, 43(2), 117–126.
Widyasari, E. M., & Zainuddin, N. (2013). The Labeling of Kanamycin Using Radionuclide of Technetium As An Agent for Early Detection of Infectious Deseases. A Scientific Journal for The Applications of Isotopes and Radiation, 9(2), 91–100
Doroudi, A., Erfani, M., Kamali-Moghaddam, M., Saadati, S. M., Ahmadi, F., Kiasat, A., … Meghdadi, H. (2014). Sensetivity and specificity of 99mTc-UBI29-41 and 67Ga-citrate scintigraphy imaging to discriminate infection lesion induced by Staphylococcus aureus and sterile inflammation lesion induced by Carrageenan in foot ’ s rat. IOSR Journal of Pharmacy, 4(11), 37–43.
Ebenhan, T., Zeevaart, J. R., Venter, J. D., Govender, T., Kruger, G. H., Jarvis, N. V, & Sathekge, M. M. (2014). Preclinical Evaluation of 68Ga-Labeled 1,4,7-Triazacyclononane-1,4,7-Triacetic Acid-Ubiquicidin as a Radioligand for PET Infection Imaging. Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine, 55(2), 1–7. http://doi.org/10.2967/jnumed.113.128397
Fazli, A., Salouti, M., Ahmadi, G., Mirshojaei, F., & Mazidi, M. (2012). Radiolabeling of Ceftriaxone with 99mTc as a Targeting Radiopharmaceutical for Staphylococcus Aureus Detection in Mouse Model, 9(2), 103–110.
Ilem-Özdemir, D., Aşikoǧlu, M., & Özkiliç, H. (2013). Radiolabeling, quality control and kit formulation of a new 99mTc-labeled antibiotic: 99mTc-doxycycline hyclate. Journal of Radioanalytical and Nuclear Chemistry, 298(3), 1635–1642. http://doi.org/10.1007/s10967-013-2547-2
Ilem-Ozdemir, D., Asikoglu, M., Ozkilic, H., Yilmaz, F., Hosgor-Limoncu, M., & Ayhan, S. (2014). 99mTc-Doxycycline hyclate: A new radiolabeled antibiotic for bacterial infection imaging. Journal of Labelled Compounds and Radiopharmaceuticals, 57(1), 36–41. http://doi.org/10.1002/jlcr.3135
Magnet, S., & Blanchard, J. S. (2005). Molecular Insights into Aminoglycoside Action and Resistance Molecular Insights into Aminoglycoside Action and Resistance. Chemical Reviews, 105(2), 477–498. http://doi.org/10.1021/cr0301088
Mirshojaei, S. F., Erfani, M., Sadat-Ebrahimi, S. E., Talebi, M. H., Haj, F., Abbasi, H., & Ir, W. S. (2010). Freeze-Dried Cold Kit for Preparation of 99mTc-Ciprofloxacin As an Infection Imaging Agent Archive of SID. Iran J Nucl Med, 18(2), 45–51.
Roohi, S., Mushtaq, A., Jehangir, M., & Malik, S. A. (2006). Synthesis, quality control and biodistribution of 99mTc- Kanamycin. Journal of Radioanalytical and Nuclear Chemistry, 267(3), 561–566. http://doi.org/10.1007/s10967-006-0087-8
Sanad, M., & Borai, E. (2014). Performance characteristics of biodistribution of 99mTc-cefprozil for in vivo infection imaging. Journal of Analytical Science and Technology, 5(1), 32. http://doi.org/10.1186/s40543-014-0032-3
Sugiharti, R. J., Widyasari, E. M., Nur, I., Dewi, U., Langsung, P., Bakteri, T., & Escherichia, D. A. N. (2015). IN-VITRO STUDY OF KANAMYCIN LABELED WITH TECHNETIUM-99m BY DIRECT METHOD TO Staphylococcus aureus AND Escherichia coli. In Proceedings of the Scientific Meeting of radioisotopes , Radiopharmaceuticals , Cyclotron and Nuclear Medicine 2015 (pp. 34–38). Bandung.
WHO. (2012). Global report for research on infectious diseases of poverty., 1–184. http://doi.org/9789241564489
Widyasari, E. M., Sriyani, M. E., Halimah, I., Wongso, H., Wibawa, T. H. A. I., & Sidik, A. (2015). EVALUATION OF PHARMACEUTICAL ASPECTS AND IN-VITRO ANTIBACTERIAL ACTIVITY OF 99MTc-KANAMYCIN DIAGNOSTIC KITS. Ganendra Journal of Nuclear Science and Technology, 18(1), 1–9.
Widyasari, E. M., Sriyani, M. E., & Nuraeni, W. (2015). STABILITY OF 99mTc-KANAMYCIN RADIOPHARMACEUTICAL FOR DETECTION OF INFECTION. In Proceedings of the Nuclear Research and Management Device Seminar (pp. 9–10).
Widyasari, E. M., Sriyani, M. E., Wibawa, T. H. A., & Nuraeni, W. (2015). Preparation of 99mTc-Kanamycin using a Direct Labeling Method. Atom Indonesia Journal, 41(3), 131–137.
Widyasari, E. M., Wibawa, T. H. A., & Nuraeni, W. (2013). PHYSICOCHEMICAL CHARACTERISTICS OF THE KANAMYCIN-LYOPHILIZED KIT. Indonesian Journal of Nuclear Science and Technology, 43(2), 117–126.
Widyasari, E. M., & Zainuddin, N. (2013). The Labeling of Kanamycin Using Radionuclide of Technetium As An Agent for Early Detection of Infectious Deseases. A Scientific Journal for The Applications of Isotopes and Radiation, 9(2), 91–100
Published
2017-02-28
How to Cite
Widyasari, E., Halimah, I., Sugiharti, R., Sriyani, M., Daruwati, I., Iswahyudi, I., Isabela, E., & Nuraeni, W. (2017). Biological Evaluation of 99mTc-Kanamycin for Infection Imaging. Indonesian Journal of Physics and Nuclear Applications, 2(1), 34-41. https://doi.org/https://doi.org/10.24246/ijpna.v2i1.34-41
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Indonesian Journal of Physics and Nuclear Applications is licensed under a Creative Commons Attribution 4.0 International License.
